Neurofilament Heavy Antibody [RT97]

• Background:

  This antibody reacts with a 200kDa protein, identified as heavy subunit of neurofilaments, or NF-H. Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H), plus alpha internexin or peripherin. Each neurofilament subunit contains a globular head, an alpha helical rod domain, and variable tail domains that differ in length and amino acid content. Neurofilament antibody stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for neurofilament antibody. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

• NCBI Gene ID:

  4744

• Swiss Prot:

  P12036

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat, Chicken, Pig

• Immunogen:

  The triton-X 100 insoluble protein fraction of rat brain was used as the immunogen for this Neurofilament Heavy antibody.

• Clonality:

  Monoclonal

• Clone:

  RT97

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Signal Transduction, Stem Cell

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  NEFH

• NCBI Official Name:

  Neurofilament, heavy polypeptide

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  NEFH, 200 kDa neurofilament protein, KIAA0845, NFH, NF-H

• Tested Applications:

  Flow, IHC

• Physical Properties:

  Liquid