Neurofilament NF-H Antibody

• Background:

  Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease.

• NCBI Gene ID:

  4744

• Swiss Prot:

  P12036

• Accession Number:

  P12036

• Host:

  Chicken

• Reactivity:

  Human, Mouse, Rat, Chicken

• Immunogen:

  Purified bovine NF-H.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Total IgY fraction

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Neurofilament NF-H antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  200

• Specificity:

  Specific for the ~200k Neurofilament H protein.

• Symbol:

  NEFH

• NCBI Official Name:

  Neurofilament, heavy polypeptide

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.

• Background Reference 02:

  Mendonca DM, Chimelli L, Martinez AM. (2005) Quantitative evidence for neurofilament heavy subunit aggregation in motor neurons of spinal cords of patients with amyotrophic lateral sclerosis. Braz J Med Biol Res. 38 (6) :925-933.

• Background Reference 03:

  Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320 (3) :156-60.

• Tested Applications:

  WB, IHC

• Protein ID:

  226726294

• Physical Properties:

  Liquid