NMDA Antibody

• Background:

  The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002) . The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996) . The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999) . The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

• NCBI Gene ID:

  24409

• Swiss Prot:

  Q00959

• Accession Number:

  Q00959

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Fusion protein from the C-terminus region of the NR2A subunit of rat NMDA receptor.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Neat Serum

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  NMDA antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  180

• Applications Notes:

  The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.

• Symbol:

  Grin2a

• NCBI Official Name:

  Glutamate receptor, ionotropic, N-methyl D-aspartate 2A

• NCBI Organism:

  Rattus norvegicus

• Background Reference 01:

  Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025.

• Background Reference 02:

  Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577.

• Background Reference 03:

  Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.

• Tested Applications:

  WB

• Protein ID:

  3915771

• Physical Properties:

  Liquid