Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody

• Background:

  The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002) . The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993) . It has been shown that phosphorylation of Ser-1480 disrupts the interaction of NR2B with the PDZ domains of PSD-95 and SAP102 and decreases surface NR2B expression in neurons (Chung et al., 2004) .

• Description:

  Boster Bio Anti-Phospho-NMDA Receptor NR2B Subunit (Ser1480) Grin2b Antibody (Catalog # P01883-4) . Tested in WB applications. This antibody reacts with Mouse, Rat.

• Phospho Site:

  Phosphorylated, Ser1480

• Synonyms:

  EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

• Gene Name:

  GRIN2B

• UniProt:

  Q00960

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser1480 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH) . Immunogen species is Rat.

• Clonality:

  Polyclonal

• Applications:

  WB

• Field of Research:

  Ligand-Gated Ion Channels, Neurodegenerative Disease, Neurology Process, Neuroscience, Neurotransmission, Receptors / Channels, Sensory System

• Purification:

  Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Dilution:

  WB: 1:1000

• Form:

  Liquid

• Function:

  NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death (By similarity) .

• Molecular Weight:

  166071 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

• Fragment:

  IgG

• Applications Notes:

  Specific for endogenous levels of the ~180 kDa NMDAR NR2B subunit protein phosphorylated at Ser1480. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

• Other Gene Names:

  Glutamate receptor ionotropic, NMDA 2B

• Subcellular Location:

  Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.

• Prediction Reactivity:

  Bovine, Canine, Chicken, Human, Primate, Xenopus

• Protein Name:

  Glutamate receptor ionotropic, NMDA 2B