Ro52 Antibody

• NCBI Gene ID:

  6737

• Swiss Prot:

  P19474

• Accession Number:

  NP_003132.2

• Host:

  Goat

• Reactivity:

  Human

• Immunogen:

  The immunogen for this antibody is: CPLNIGSQGSTDY

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• Concentration:

  500 ug/mL

• Buffer:

  Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and store at -20˚ C. Minimize freezing and thawing.

• Applications Notes:

  Peptide ELISA: Western Blot:Immunofluorescence: Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Additional validation:

• Symbol:

  TRIM21

• NCBI Official Name:

  Tripartite motif containing 21

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chan EK, Di Donato F, Hamel JC, Tseng CE, Buyon JP. 52-kD SS-A/Ro: genomic structure and identification of an alternatively spliced transcript encoding a novel leucine zipper-minus autoantigen expressed in fetal and adult heart. J Exp Med. 1995 Oct 1;182 (4) :983-92.

• Other Product Names:

  TRIM21, tripartite motif-containing 21, RNF81, RO52, SSA, SSA1, 52kD Ro/SSA autoantigen, 52kDa ribonucleoprotein autoantigen SS-A/Ro, Sicca syndrome antigen A, Sjogren syndrome antigen A1 (52kD, ribonucleoprotein autoantigen SS-A/Ro), Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro), tripartite motif protein TRIM21, tripartite motif protein 21

• Tested Applications:

  ELISA, WB, IF, Flow

• Protein ID:

  15208660

• Physical Properties:

  Liquid