PCNA Antibody

• NCBI Gene ID:

  5111

• Swiss Prot:

  P12004

• Accession Number:

  NP_002583.1

• Host:

  Goat

• Reactivity:

  Human, Mouse, Rat, Pig

• Immunogen:

  The immunogen for this antibody is: C-NGNIKLSQTSNVD

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Transcription

• Purification:

  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

• Concentration:

  500 ug/mL

• Homology:

  Expected Species Reactivity based on sequence homology: Dog, Cow

• Buffer:

  Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and store at -20˚ C. Minimize freezing and thawing.

• Calculated Molecular Weight:

  Approx 30kDa

• Specificity:

  Reported variants represent identical protein: NP_872590.1, NP_002583.1

• Applications Notes:

  Peptide ELISA: Western Blot:Enzyme immunoassay: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Additional validation:

• Symbol:

  PCNA

• NCBI Official Name:

  Proliferating cell nuclear antigen

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Jones MJ, Colnaghi L, Huang TT.Dysregulation of DNA polymerase κ recruitment to replication forks results in genomic instability.

• Other Product Names:

  PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin

• Tested Applications:

  ELISA, WB, EIA

• Protein ID:

  4505641

• Physical Properties:

  Liquid