ANGPTL4 Antibody

• Background:

  ANGPTL4 mainly expressed in endothelial cells (hypoxia-induced) . Regulates angiogenesis and modulates tumorgenesis and directly regulates lipid, glucose, and energy metabolism. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. ANGPTL4 is a protein consisting of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain (FLD) . Both domains have distinct biological functions. The coiled-coil domain is responsible for the inhibitory effects on lipoprotein lipase (LPL) converting the active form of LPL into an inactive form, and the FLD domain mediates its antiangiogenic functions. The coiled coil and the FLD domains are separated by a short linker that can be cleaved after secretion. ANGPTL4 appears on the cell surface as the full-length form, where it can be released by heparin treatment. ANGPTL4 protein is then proteolytically cleaved by proprotein convertases (PCs), including furin, PC5/6, paired basic amino acid-cleaving enzyme 4, and PC7.

• NCBI Gene ID:

  51129

• Swiss Prot:

  Q9BY76

• Accession Number:

  25008123

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Coiled-coil domain of recombinant human ANGPTL4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 6 months after receipt when stored at -20˚ C.

• Specificity:

  Recognizes the coiled-coil domain of human ANGPTL4. Detects a band of ~19kDa by Western blot. Weakly cross-reacts with human ANGPTL2 (coiled-coil domain), ANGPTL3 and ANGPTL5 (coiled-coil domain) .

• Applications Notes:

  ELISA: (direct and indirect: 1:2,000-1:5,000) . Western Blot: (1:2,000-1:5,000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

• Symbol:

  ANGPTL4

• NCBI Official Name:

  Angiopoietin like 4

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Angiopoietin-like Protein 4; FIAF; Fasting-induced Adipose Factor; HFARP; Hepatic Fibrinogen/Angiopoietin-related Protein

• Tested Applications:

  ELISA, WB

• Protein ID:

  Q9BY76

• Physical Properties:

  Liquid