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Apaf-1 Antibody [2E12]

CAT: 0223-36-175Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0223-36-175Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Apaf-1 is a cytoplasmic protein that forms one of the central hubs in the apoptosis regulatory network. It contains (from the N terminal) a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome, mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.
NCBI Gene ID
317
Swiss Prot
O14727
Accession Number
2330015
Host
Rat
Reactivity
Human
Immunogen
Recombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
Clonality
Monoclonal
Clone
2E12
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Apoptosis, Cancer
Purification
>95% (SDS-PAGE)
Concentration
1 mg/ml
Buffer
Liquid. In PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Stable for at least 1 year after receipt when stored at -20˚ C.
Fragment
IgG2a, kappa
Specificity
Recognizes the CARD domain of human Apaf-1. Does not cross-react with mouse or rat Apaf-1.
Applications Notes
Immunohistochemistry: (paraffin sections, 10ug/ml) . Immunohistochemistry: (EM on frozen sections, see G. Hausmann, et al.; (2000) ) . Immunoprecipitation: (1:1000-1:2000) . Western Blot: (1:1000-1:2000) . Optimal conditions must be determined individually for each application.
Symbol
APAF1
NCBI Official Name
Apoptotic peptidase activating factor 1
NCBI Organism
Homo sapiens
Other Product Names
Apoptosis Protease-activating Factor 1
Tested Applications
ELISA, ICC, IHC, IP, WB
Protein ID
AAC51678
Physical Properties
Liquid

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MC-287Apaf-1 Monoclonal Antibody [2E12]
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NBP2-80092Rat Monoclonal APAF-1 Antibody (2E12)
NBP2-80092HRat Monoclonal APAF-1 Antibody (2E12) [HRP]
NBP2-80092FRat Monoclonal APAF-1 Antibody (2E12) [FITC]
NBP2-80092APCRat Monoclonal APAF-1 Antibody (2E12) [Allophycocyanin]
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NBP2-80092BRat Monoclonal APAF-1 Antibody (2E12) [Biotin]
NBP2-80092PERat Monoclonal APAF-1 Antibody (2E12) [PE]
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