APAF1 Antibody

• Background :

  Apoptotic protease activating factor 1. Apaf-1 is a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC) . Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.

• NCBI Gene ID :

  317

• Swiss Prot :

  O14727

• Accession Number :

  NP_863658.1

• Host :

  Chicken

• Reactivity :

  Human, Mouse

• Immunogen :

  307-388

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Apoptosis, Cancer

• Purification :

  Antigen affinity-purified

• Concentration :

  1 mg/mL

• Buffer :

  Phosphate-Buffered Saline. No preservatives added.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  APAF1 antibody can be stored at 4˚ C for short term (weeks) . Long term storage should be at -20˚ C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  141.8 kDa (calculated)

• Applications Notes :

  Apaf-1 antibody can be used for the detection of Apaf-1 by Western blot, may also work for IHC and ICC.

• Symbol :

  APAF1

• NCBI Official Name :

  Apoptotic peptidase activating factor 1

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Cecconi, F., Alvarez-Bolado, G., Meyer, B. I., Roth, K. A. and Gruss, P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94 (6), 727-737 (1998)

• Other Product Names :

  KIAA0413, Apoptotic protease-activating factor 1APAF-1, CED4, APAF-1

• Tested Applications :

  WB

• Protein ID :

  32483361

• Physical Properties :

  Liquid