Nampt Antibody [OMNI379]

• Background:

  Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+) . Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt) . While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

• NCBI Gene ID:

  10135

• Swiss Prot:

  P43490

• Accession Number:

  5031977

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human Nampt (visfatin/PBEF) .

• Clonality:

  Monoclonal

• Clone:

  OMNI379

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity

• Concentration:

  1 mg/ml

• Buffer:

  Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable for at least 1 year after receipt when stored at -20˚ C.

• Fragment:

  IgG2a, kappa

• Specificity:

  Recognizes human, mouse and rat Nampt.

• Applications Notes:

  ELISA: (direct or indirect: 1:2,000-1:10,000) . Flow Cytometry: (1:1,000) . Immunocytochemistry: (1:500-1:1,000) . Immunohistochemistry: (paraffin sections, 1:100-1:500) . Immunoprecipitation: (1:500-1:1,000) . Western blot: (1:2,000-1:5,000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature) . Optimal conditions should be determined individually for each application.

• Symbol:

  NAMPT

• NCBI Official Name:

  Nicotinamide phosphoribosyltransferase

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase

• Tested Applications:

  ELISA, Flow, ICC, IHC, IP, WB

• Protein ID:

  NP_005737

• Physical Properties:

  Liquid