SETDB1 Antibody

• Background:

  The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su (var) 3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities.

• NCBI Gene ID:

  9869

• Swiss Prot:

  Q15047

• Accession Number:

  Q15047

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SETDB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1193-1225 amino acids from the C-terminal region of human SETDB1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Zebrafish, Xenopus

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  143 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  SETDB1

• NCBI Official Name:

  Histone-lysine N-methyltransferase SETDB1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ichimura, T., et al., J. Biol. Chem. 280 (14) :13928-13935 (2005) .

• Background Reference 02:

  Sarraf, S.A., et al., Mol. Cell 15 (4) :595-605 (2004) .

• Background Reference 03:

  Wang, H., et al., Mol. Cell 12 (2) :475-487 (2003) .

• Background Reference 04:

  Schultz, D.C., et al., Genes Dev. 16 (8) :919-932 (2002) .

• Other Product Names:

  Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, ESET, Histone H3-K9 methyltransferase 4, H3-K9-HMTase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SETDB1, KIAA0067, KMT1E

• Tested Applications:

  WB

• Protein ID:

  25091210

• Physical Properties:

  Liquid