ACF Antibody

• Background:

  Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events.

• NCBI Gene ID:

  29974

• Swiss Prot:

  Q9NQ94

• Accession Number:

  Q9NQ94

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ACF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 394-423 amino acids from the C-terminal region of human ACF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  65 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  A1CF

• NCBI Official Name:

  APOBEC1 complementation factor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Galloway, C.A., et al. Biochem. Biophys. Res. Commun. 391 (1) :659-663 (2010)

• Background Reference 02:

  Blanc, V., et al. Mol. Cell. Biol. 25 (16) :7260-7269 (2005)

• Background Reference 03:

  Deloukas, P., et al. Nature 429 (6990) :375-381 (2004)

• Background Reference 04:

  Xie, K., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (21) :8114-8119 (2004)

• Other Product Names:

  APOBEC1 complementation factor, APOBEC1-stimulating protein, A1CF, ACF, ASP {ECO:0000312|EMBL:CAB947541}

• Tested Applications:

  WB, IHC-P, Flow

• Protein ID:

  74761651

• Physical Properties:

  Liquid