Gp100 Antibody [SPM286]

• Background:

  By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

• NCBI Gene ID:

  6490

• Swiss Prot:

  P40967

• Host:

  Mouse

• Reactivity:

  Human, Horse

• Immunogen:

  Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

• Clonality:

  Monoclonal

• Clone:

  SPM286

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Obesity, Signal Transduction

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG2b, kappa

• Specificity:

  Does not react with rat

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

• Symbol:

  PMEL

• NCBI Official Name:

  Premelanosome protein

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Melanocyte protein PMEL, ME20-M, ME20M, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, P1, P100, Premelanosome protein, Silver locus protein homolog, M-alpha, 95 kDa melanocyte-specific secreted glycoprotein, P26, Secreted melanoma-associated ME20 antigen, ME20-S, ME20S, M-beta, PMEL, D12S53E, PMEL17, SILV

• Tested Applications:

  Flow, IF, IHC

• Physical Properties:

  Liquid