CD35 Antibody [SPM554]

• Background:

  Recognizes a protein of 210-220kDa, which is identified as the Complement receptor 1 or CD35. This mAb is specific for a site in CR1 that is distal from the C3b-binding site, so that it is unable to block CR1/CD35 activity. It is highly specific and shows no cross-reaction with CR2/CD21. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

• NCBI Gene ID:

  1378

• Swiss Prot:

  P17927

• Host:

  Mouse

• Reactivity:

  Human, Baboon, Cynomolgus Monkey, Rhesus Monkey

• Immunogen:

  Human monocytes were used as the immunogen for this CD35 antibody.

• Clonality:

  Monoclonal

• Clone:

  SPM554

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology, Stem Cell

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Applications Notes:

  1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  CR1

• NCBI Official Name:

  Complement component (3b/4b) receptor 1 (Knops blood group)

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  CR1, C3b/C4b receptor, C3-binding protein, CD35, C3BR, Knops blood group antigen, C4BR, CD35 antigen, Complement receptor type 1, KN

• Tested Applications:

  Flow, IF, IHC

• Physical Properties:

  Liquid