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Nuclear Antigen Antibody

CAT: 0800-V7192SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7192SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
Not Known
Host
Mouse
Reactivity
Human, Rat
Immunogen
Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NUCM1-1
Applications
IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the Nuclear Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Nuclear Antigen antibody is available for research use only.
Storage Conditions
Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Nuclei
Image Legend
IHC testing of FFPE rat lung with Nuclear Antigen antibody (clone NUCM1-1) . FFPE testing requires sections to be boiled in pH 9 10mM Tris with 1mM EDTA for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.

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