CD20 Antibody [B9E9]

This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12) . The antibody recognizes the extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This antibody can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, Bcell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of Bcells and its expression is restricted to normal and neoplastic Bcells, being absent from all other leukocytes and tissues. It is expressed by pre Bcells and persists during all stages of Bcell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting Bcells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in Bcell activation and cell cycle progression.

931

P11836

Human

The lymphoblastoid cell line Daudi was used as the immunogen for this CD20 antibody.

B9E9

Unconjugated

Primary Antibodies

Cancer, Immunology, Signal Transduction

Protein G affinity chromatography

0.2 mg/mL

PBS with 0.1 mg/ml BSA and 0.05% sodium azide

None

Blue Ice

Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

IgG2a, kappa

MS4A1

Membrane spanning 4-domains A1

Homo sapiens

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20, MS4A1, CD20

Functional Assays, Flow, IF