Bcl-2 Antibody [100/D5]

• Background:

  This antibody recognizes a protein of 25-26kDa, identified as the Bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax. Expression of Bcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis) . In most follicular lymphomas, neoplastic germinal centers express high levels of the protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, Bcl-2 antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express Bcl-2 and the small number in which the neoplastic cells are negative.

• NCBI Gene ID:

  596

• Swiss Prot:

  P10415

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids GAAPAPGIFSSQPG (41-54) of the human protein were used as the immunogen for this Bcl-2 antibody.

• Clonality:

  Monoclonal

• Clone:

  100/D5

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Apoptosis, Autophagy, Cancer

• Purification:

  Protein G affinity chromatography

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS with 0.1 mg/ml BSA and 0.05% sodium azide

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Aliquot and Store at 2-8˚ C. Avoid freez-thaw cycles.

• Fragment:

  IgG1, kappa

• Specificity:

  Does not react with mouse and rat

• Applications Notes:

  1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes. 2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• Symbol:

  BCL2

• NCBI Official Name:

  B-cell CLL/lymphoma 2

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2

• Tested Applications:

  WB, Flow, IHC, ELISA, IF, IP

• Physical Properties:

  Liquid