Bcl-2 Antibody

CAT: 0223-3337-01Size: 0.02 mgDry Ice: NoHazardous: No
CAT#:0223-3337-01Size:0.02 mg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.
NCBI Gene ID
596
Swiss Prot
P10415
Accession Number
AAH27258
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
3337
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Validation
Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain. Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3335 3337
Field of Research
Apoptosis, Neuroscience, Autophagy, Cancer
Purification
Bcl-2 Antibody is affinity chromatography purified via peptide column.
Positive Control
Cat. No. 1288 - A20 Cell Lysate
Concentration
1 mg/mL
Homology
Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (93%), Chicken: (86%)
Buffer
Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Bcl-2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Fragment
IgG
Isoform
Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD) . Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD) . Rat Bcl-2 has one isoform (236aa, 27kD) . 3337 can detect all isoforms of human, mouse and rat
Symbol
BCL2
Positive Control 2
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 3
Cat. No. 1208 - THP-1 Cell Lysate
NCBI Official Name
B-cell CLL/lymphoma 2
NCBI Organism
Homo sapiens
Background Reference 01
Lockshin et al. Cell Death Differ. 2000; 7:2-7.
Background Reference 02
Cory et al. Oncogene 2003; 22:8590-607.
Background Reference 03
Heiser et al. Exp. Geron. 2004; 39:1125-35.
Background Reference 04
Hockenbery et al. Nature 1990; 348:334-6.
Other Product Names
Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2
Tested Applications
ELISA, WB, ICC
Protein ID
20072668
Physical Properties
Liquid

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