Bcl-2 Antibody

• Background:

  Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

• NCBI Gene ID:

  596

• Swiss Prot:

  P10415

• Accession Number:

  AAH27258

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  3335

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Validation:

  Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain. Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies. Independent Antibody Validation in Cell lines3335 3337

• Field of Research:

  Apoptosis, Autophagy, Cancer

• Purification:

  Bcl-2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1288 - A20 Cell Lysate

• Concentration:

  1 mg/mL

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (100%), Chicken: (93%)

• Buffer:

  Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Bcl-2antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Isoform:

  Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD), and isoform 2 (205aa, 22kD) . Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD), and isoform 2 (199aa, 22kD) . Rat Bcl-2 has one isoform (236aa, 27kD) . 3337 can detect all isoforms of human, mouse and rat

• Symbol:

  BCL2

• Positive Control 2:

  Cat. No. 1305 - Human Kidney Tissue Lysate

• Positive Control 3:

  Cat. No. 10-401 - Human Kidney Tissue Slide

• Positive Control 4:

  Cat. No. 1219 – MCF-7 Cell Lysate

• Positive Control 5:

  Cat. No. 1208 – THP-1 Cell Lysate

• NCBI Official Name:

  B-cell CLL/lymphoma 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lockshin et al. Cell Death Differ. 2000; 7:2-7.

• Background Reference 02:

  Cory et al. Oncogene 2003; 22:8590-607.

• Background Reference 03:

  Heiser et al. Exp. Geron. 2004; 39:1125-35.

• Background Reference 04:

  Hockenbery et al. Nature 1990; 348:334-6.

• Other Product Names:

  Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  20072668

• Physical Properties:

  Liquid