PDE Antibody

• Background:

  2'-PDE is an enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. 2'-PDE also cleaves 3',5'-phosphodiester bond of oligoadenylates. 2'-PDE play a role as a negative regulator of the 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs) . Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system.

• NCBI Gene ID:

  201626

• Swiss Prot:

  Q6L8Q7

• Accession Number:

  NP_808881

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDE.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  OVCAR-3 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDE antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  67 kDa

• Applications Notes:

  PDE antibody can be used for detection of PDE by ELISA at 1:1562500. PDE antibody can be used for detection of PDE by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PDE12

• NCBI Official Name:

  Phosphodiesterase 12

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kubota, K., (2004) J. Biol. Chem. 279 (36), 37832-37841 .

• Other Product Names:

  2'-PDE, 2'-PDE

• Tested Applications:

  ELISA, WB

• Protein ID:

  189027129

• Physical Properties:

  Liquid