NFKBIA Antibody

• Background:

  NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) .

• NCBI Gene ID:

  4792

• Swiss Prot:

  P25963

• Accession Number:

  NP_065390

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFKBIA.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer, Signal Transduction, Immunology, Apoptosis

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. XBL-10410 - Fetal Lung Tissue Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NFKBIA antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  36 kDa

• Applications Notes:

  NFKBIA antibody can be used for detection of NFKBIA by ELISA at 1:1562500. NFKBIA antibody can be used for detection of NFKBIA by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NFKBIA

• NCBI Official Name:

  Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Janssen, R., et al., (2004) J. Exp. Med. 200 (5), 559-568.

• Other Product Names:

  NFKBIA, IKBA, MAD-3, NFKBI

• Tested Applications:

  ELISA, WB

• Protein ID:

  10092619

• Physical Properties:

  Liquid