TFAP2A Antibody

• Background:

  TFAP2A is a sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limbs and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2 alpha is the only AP-2 protein required for early morphogenesis of the lens vesicleAP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.

• NCBI Gene ID:

  7020

• Swiss Prot:

  P05549

• Accession Number:

  NP_003211

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TFAP2A.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer, Signal Transduction

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TFAP2A antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  48 kDa

• Applications Notes:

  TFAP2A antibody can be used for detection of TFAP2A by ELISA at 1:312500. TFAP2A antibody can be used for detection of TFAP2A by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TFAP2A

• NCBI Official Name:

  Transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Li, M., et al., (2006) Int. J. Cancer 118 (4), 802-811.

• Other Product Names:

  TFAP2A, AP-2, BOFS, AP2TF, TFAP2, AP-2alpha

• Tested Applications:

  ELISA, WB

• Protein ID:

  4507441

• Physical Properties:

  Liquid