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TCF4 Antibody

CAT: 0223-31-179Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-31-179Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. TCF4 is known to produce multiple transcripts; however as the complete structure is only known for the transcript that encodes the b isoform, that is the variant presented here.
NCBI Gene ID
6925
Swiss Prot
P15884
Accession Number
NP_003190
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF4.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TCF4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
71 kDa
Applications Notes
TCF4 antibody can be used for detection of TCF4 by ELISA at 1:62500. TCF4 antibody can be used for detection of TCF4 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
TCF4
NCBI Official Name
Transcription factor 4
NCBI Organism
Homo sapiens
Background Reference 01
Raurell, I., (2006) J. Biol. Chem. 281 (3), 1401-1411.
Other Product Names
TCF4, E2-2, ITF2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, PTHS, ITF-2, SEF-2, TCF-4, SEF2-1D, bHLHb19
Tested Applications
ELISA, WB
Protein ID
4507399
Physical Properties
Liquid

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