TCF4 Antibody

• Background:

  TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well.TCF4 encodes transcription factor 4, a basic helix-turn-helix transcription factor. The protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. TCF4 is expressed predominantly in pre-B-cells, although it is found in other tissues as well. TCF4 is known to produce multiple transcripts; however as the complete structure is only known for the transcript that encodes the b isoform, that is the variant presented here.

• NCBI Gene ID:

  6925

• Swiss Prot:

  P15884

• Accession Number:

  NP_003190

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TCF4.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TCF4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  71 kDa

• Applications Notes:

  TCF4 antibody can be used for detection of TCF4 by ELISA at 1:62500. TCF4 antibody can be used for detection of TCF4 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TCF4

• NCBI Official Name:

  Transcription factor 4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Raurell, I., (2006) J. Biol. Chem. 281 (3), 1401-1411.

• Other Product Names:

  TCF4, E2-2, ITF2, SEF2, SEF2-1, SEF2-1A, SEF2-1B, PTHS, ITF-2, SEF-2, TCF-4, SEF2-1D, bHLHb19

• Tested Applications:

  ELISA, WB

• Protein ID:

  4507399

• Physical Properties:

  Liquid