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ECT2 Antibody

CAT: 0223-30-811Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-30-811Size:100 µL
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PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
ECT2 is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of ECT2 is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
NCBI Gene ID
1894
Swiss Prot
Q9H8V3
Accession Number
NP_060568
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ECT2.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Signal Transduction, Immunology
Purification
Antibody is purified by peptide affinity chromatography method.
Positive Control
Cat. No. 1201 - HeLa Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ECT2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
100 kDa
Applications Notes
ECT2 antibody can be used for detection of ECT2 by ELISA at 1:12500. ECT2 antibody can be used for detection of ECT2 by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
ECT2
NCBI Official Name
Epithelial cell transforming sequence 2 oncogene
NCBI Organism
Homo sapiens
Background Reference 01
Nagasaki, A., (2007) Plasmid 58 (2), 190-194.
Other Product Names
ECT2, FLJ10461, MGC138291, ARHGEF31
Tested Applications
ELISA, WB
Protein ID
21735572
Physical Properties
Liquid

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