TYMS Antibody

TYMS catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs.Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occuring antisense transcript rTSalpha (GeneID:55556) vary inversely when cell-growth progresses from late-log to plateau phase. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-47 CR601528.1 1-47 48-1099 X02308.1 14-1065 1100-1603 BC083512.1 1067-1570

7298

P04818

NP_001062

Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TYMS.

Unconjugated

Primary Antibodies

Cancer

Antibody is purified by peptide affinity chromatography method.

Cat. No. 1205 - Jurkat Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TYMS antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

36 kDa

TYMS antibody can be used for detection of TYMS by ELISA at 1:12500. TYMS antibody can be used for detection of TYMS by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

TYMS

Thymidylate synthetase

Homo sapiens

Ikeda, S., (2008) Am. J. Gastroenterol. 103 (6), 1476-1487.

TYMS, HsT422, MGC88736, TMS, TS, TSase, HST422

ELISA, WB

4507751