GOT1 Antibody

• Background:

  Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

• NCBI Gene ID:

  2805

• Swiss Prot:

  P17174

• Accession Number:

  NP_002070

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GOT1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GOT1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  46 kDa

• Applications Notes:

  GOT1 antibody can be used for detection of GOT1 by ELISA at 1:312500. GOT1 antibody can be used for detection of GOT1 by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  GOT1

• NCBI Official Name:

  Glutamic-oxaloacetic transaminase 1, soluble

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Inoue, K., Diabetes Res. Clin. Pract. 79 (3), E4-E7 (2008) .

• Other Product Names:

  GOT1, GIG18, cCAT, cAspAT, ASTQTL1

• Tested Applications:

  ELISA, WB

• Protein ID:

  4504067

• Physical Properties:

  Liquid