DDOST Antibody

• Background:

  DDOST is a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. DDOST is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

• NCBI Gene ID:

  1650

• Swiss Prot:

  Q5VWA5

• Accession Number:

  NP_005207

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DDOST.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Signal Transduction

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DDOST antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  46 kDa

• Applications Notes:

  DDOST antibody can be used for detection of DDOST by ELISA at 1:62500. DDOST antibody can be used for detection of DDOST by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  DDOST

• NCBI Official Name:

  Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shibatani, T., (2005) Biochemistry 44 (16), 5982-5992.

• Other Product Names:

  DDOST, AGE-R1, KIAA0115, MGC2191, OK/SW-cl.45, OST, OST48, WBP1, AGER1, CDG1R, OKSWcl45

• Tested Applications:

  ELISA, WB

• Protein ID:

  20070197

• Physical Properties:

  Liquid