GCDH Antibody

• Background :

  GCDH belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits.The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.

• NCBI Gene ID :

  2639

• Swiss Prot :

  Q92947

• Accession Number :

  NP_000150

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GCDH.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GCDH antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  48 kDa

• Applications Notes :

  GCDH antibody can be used for detection of GCDH by ELISA at 1:1562500. GCDH antibody can be used for detection of GCDH by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  GCDH

• NCBI Official Name :

  Glutaryl-CoA dehydrogenase

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Kimura, K., (2006) Genome Res. 16 (1), 55-65.

• Other Product Names :

  GCDH, ACAD5, GCD

• Tested Applications :

  ELISA, WB, IHC

• Protein ID :

  4503943

• Physical Properties :

  Liquid