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GCDH Antibody

CAT: 0223-29-884Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-29-884Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
GCDH belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits.The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
NCBI Gene ID
2639
Swiss Prot
Q92947
Accession Number
NP_000150
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Zebrafish
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GCDH.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store GCDH antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
48 kDa
Applications Notes
GCDH antibody can be used for detection of GCDH by ELISA at 1:1562500. GCDH antibody can be used for detection of GCDH by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
GCDH
NCBI Official Name
Glutaryl-CoA dehydrogenase
NCBI Organism
Homo sapiens
Background Reference 01
Kimura, K., (2006) Genome Res. 16 (1), 55-65.
Other Product Names
GCDH, ACAD5, GCD
Tested Applications
ELISA, WB, IHC
Protein ID
4503943
Physical Properties
Liquid

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