AMFR Antibody

• Background :

  Autocrine motility factor is a tumor motility-stimulating protein secreted by tumor cells. AMFR is a glycosylated transmembrane protein and a receptor for autocrine motility factor. The receptor, which shows some sequence similarity to tumor protein p53, is localized to the leading and trailing edges of carcinoma cells.The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family (Gray et al., 2000) .

• NCBI Gene ID :

  267

• Swiss Prot :

  Q6PGR1

• Accession Number :

  AAH56869

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat, Dog

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AMFR.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Apoptosis, Homeostasis

• Purification :

  Antibody is purified by protein A chromatography method.

• Positive Control :

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store AMFR antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  53 kDa, 53 kDa

• Applications Notes :

  AMFR antibody can be used for detection of AMFR by ELISA at 1:312500. AMFR antibody can be used for detection of AMFR by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  AMFR

• NCBI Official Name :

  Autocrine motility factor receptor, E3 ubiquitin protein ligase

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Lehner, B. (2004) Genome Res. 14 (7), 1315-1323.

• Other Product Names :

  AMFR, GP78, RNF45

• Tested Applications :

  ELISA, WB

• Protein ID :

  34784904

• Physical Properties :

  Liquid