PRKCZ Antibody

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

5590

Q05513

NP_001028753

Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRKCZ.

Unconjugated

Primary Antibodies

Membrane, Cancer

Antibody is purified by peptide affinity chromatography method.

Cat. No. 1205 - Jurkat Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PRKCZ antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

45 kDa, 47 kDa, 68 kDa

PRKCZ antibody can be used for detection of PRKCZ by ELISA at 1:62500. PRKCZ antibody can be used for detection of PRKCZ by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

PRKCZ

Protein kinase C, zeta

Homo sapiens

Xie, Z., (2006) J. Biol. Chem. 281 (10), 6366-6375.

PRKCZ, PKC2, PKC-ZETA

ELISA, WB

75709226