TNNI1 Antibody

• Background:

  Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Each troponin-tropomyosin complex contains 1 molecule from each of the 3 troponins: T, C, and I. The troponin I (TNI) subfamily contains three genes: TNI-skeletal-fast-twitch, TNI-skeletal-slow-twitch, and TNI-cardiac. TNNI1 prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.

• NCBI Gene ID:

  7135

• Swiss Prot:

  P19237

• Accession Number:

  NP_003272

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TNNI1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TNNI1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  21 kDa

• Applications Notes:

  TNNI1 antibody can be used for detection of TNNI1 by ELISA at 1:62500. TNNI1 antibody can be used for detection of TNNI1 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TNNI1

• NCBI Official Name:

  Troponin I type 1 (skeletal, slow)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ralli, S., (2005) Am. Heart J. 150 (6), 1220-1227.

• Other Product Names:

  TNNI1, DKFZp451O223, SSTNI, TNN1

• Tested Applications:

  ELISA, WB

• Protein ID:

  56682969

• Physical Properties:

  Liquid