DLAT Antibody

• Background:

  DLAT is dihydrolipoamide acetyltransferase, the E2 subunit of the mammalian pyruvate dehydrogenase complex of the inner mitochondrial membrane. Patients with primary biliary cirrhosis show autoantibodies to DLAT.The DLAT gene encodes dihydrolipoamide acetyltransferase (EC 2.3.1.12), the E2 subunit of the mammalian pyruvate dehydrogenase complex (PDC; EC 1.2.4.1) of the inner mitochondrial membrane. Patients with primary biliary cirrhosis (PBC; MIM 109720) show autoantibodies to DLAT.

• NCBI Gene ID:

  1737

• Swiss Prot:

  Q86YI5

• Accession Number:

  NP_001922

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human DLAT.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store DLAT antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  71 kDa

• Applications Notes:

  DLAT antibody can be used for detection of DLAT by ELISA at 1:312500. DLAT antibody can be used for detection of DLAT by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  DLAT

• NCBI Official Name:

  Dihydrolipoamide S-acetyltransferase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Head, R.A., (2005) Ann. Neurol. 58 (2), 234-241.

• Other Product Names:

  DLAT, DLTA, PDC-E2, PDCE2

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  31711992

• Physical Properties:

  Liquid