PKLR Antibody

• Background:

  PKLR is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA) .The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA) . Alternatively spliced transcript variants encoding distinct isoforms have been described.

• NCBI Gene ID:

  5313

• Swiss Prot:

  P30613-2

• Accession Number:

  NP_870986

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, C. elegans, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PKLR.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PKLR antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  58 kDa, 62 kDa

• Applications Notes:

  PKLR antibody can be used for detection of PKLR by ELISA at 1:62500. PKLR antibody can be used for detection of PKLR by western blot at 5.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PKLR

• NCBI Official Name:

  Pyruvate kinase, liver and RBC

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pendergrass, D.C., (2006) IUBMB Life 58 (1), 31-38.

• Other Product Names:

  PKLR, PK1, PKL, RPK, PKR, PKRL

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  32967597

• Physical Properties:

  Liquid