MSH2 Antibody

• Background:

  MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) . When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

• NCBI Gene ID:

  4436

• Swiss Prot:

  P43246

• Accession Number:

  AAY15096

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MSH2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Cancer, Apoptosis

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MSH2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  64 kDa, 105 kDa

• Applications Notes:

  MSH2 antibody can be used for detection of MSH2 by ELISA at 1:62500. MSH2 antibody can be used for detection of MSH2 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MSH2

• NCBI Official Name:

  MutS homolog 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hoss, M. EMBO J. (1999) 18 (13), 3868-3875.

• Other Product Names:

  MSH2, FCC1, COCA1, HNPCC, LCFS2, HNPCC1

• Tested Applications:

  ELISA, WB

• Protein ID:

  62822548

• Physical Properties:

  Liquid