CARF Antibody

• Background:

  CARF was first cloned as a novel binding partner of ARF from a yeast-interactive screen. CARF and ARF colocalize in the perinucleolar region and have a collaborative function. In the nucleoplasm, CARF interacts with p53 and enhances its function. The p53 downregulates CARF in a negative feedback regulatory loop and may also involve p53 antagonist HDM2.

• NCBI Gene ID:

  55602

• Swiss Prot:

  Q9NXV6

• Accession Number:

  NP_060102

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CARF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store CARF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  64 kDa

• Applications Notes:

  CARF antibody can be used for detection of CARF by ELISA at 1:312500. CARF antibody can be used for detection of CARF by western blot at 0.3125 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  CDKN2AIP

• NCBI Official Name:

  CDKN2A interacting protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hasan, M.K., (2002) J. Biol. Chem. 277 (40), 37765-37770.

• Other Product Names:

  CARF, CARF

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  8923040

• Physical Properties:

  Liquid