PPIE Antibody

CAT: 0223-29-363Size: 100 µLDry Ice: NoHazardous: No
CAT#:0223-29-363Size:100 µL
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Background
PPIE is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activityThe protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed.
NCBI Gene ID
10450
Swiss Prot
Q9UNP9
Accession Number
NP_982282
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Zebrafish, Drosophila
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPIE.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1205 - Jurkat Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PPIE antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
26 kDa, 33 kDa, 33 kDa
Applications Notes
PPIE antibody can be used for detection of PPIE by ELISA at 1:312500. PPIE antibody can be used for detection of PPIE by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
PPIE
NCBI Official Name
Peptidylprolyl isomerase E (cyclophilin E)
NCBI Organism
Homo sapiens
Background Reference 01
Fair, K., (2001) Mol. Cell. Biol. 21 (10), 3589-3597.
Other Product Names
PPIE, CYP33, CYP-33
Tested Applications
ELISA, WB
Protein ID
45439318
Physical Properties
Liquid

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