PTBP1 Antibody

PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA) . These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs) . The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA) . These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.

5725

P26599

NP_114368

Human

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PTBP1.

Unconjugated

Primary Antibodies

Cancer

Antibody is purified by peptide affinity chromatography method.

Cat. No. 1224 - Daudi Cell Lysate

Batch dependent

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

None

Blue Ice

For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PTBP1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

58 kDa, 60 kDa, 59 kDa

PTBP1 antibody can be used for detection of PTBP1 by ELISA at 1:12500. PTBP1 antibody can be used for detection of PTBP1 by western blot at 1.0 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

PTBP1

Polypyrimidine tract binding protein 1

Homo sapiens

Robinson, F. (2006) J. Biol. Chem. 281 (2), 800-806.

PTBP1, HNRNPI, HNRPI, MGC10830, MGC8461, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB, HNRNP-I

ELISA, WB

14165466