PIGF Antibody

• Background:

  PIGF is a protein involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. PIGF and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. This gene encodes a protein involved in glycosylphosphatidylinositol (GPI) -anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described.

• NCBI Gene ID:

  5281

• Swiss Prot:

  Q07326

• Accession Number:

  NP_002634

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIGF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Membrane, Signal Transduction

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PIGF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  25 kDa

• Applications Notes:

  PIGF antibody can be used for detection of PIGF by ELISA at 1:12500. PIGF antibody can be used for detection of PIGF by western blot at 1 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  PIGF

• NCBI Official Name:

  Phosphatidylinositol glycan anchor biosynthesis, class F

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Mohammed, K.A., Am. J. Physiol. Lung Cell Mol. Physiol. 292 (2), L559-L566 (2007) .

• Other Product Names:

  PIGF, MGC32646, MGC33136

• Tested Applications:

  ELISA, WB

• Protein ID:

  4505797

• Physical Properties:

  Liquid