AARS Antibody

• Background :

  The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

• NCBI Gene ID :

  16

• Swiss Prot :

  P49588

• Accession Number :

  NP_001596

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AARS.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  Antibody is purified by peptide affinity chromatography method.

• Positive Control :

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration :

  Batch dependent

• Buffer :

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store AARS antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight :

  107 kDa

• Applications Notes :

  AARS antibody can be used for detection of AARS by ELISA at 1:312500. AARS antibody can be used for detection of AARS by western blot at 0.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol :

  AARS

• NCBI Official Name :

  Alanyl-tRNA synthetase

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Olsen, J.V., (2006) Cell 127 (3), 635-648.

• Other Product Names :

  AARS, CMT2N

• Tested Applications :

  ELISA, WB

• Protein ID :

  109148542

• Physical Properties :

  Liquid