PDCD8 Antibody

• Background:

  AIFM1 (PDCD8) is a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, AIFM1 induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells that is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it effects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Three alternative transcripts encoding different isoforms have been identified for this gene.

• NCBI Gene ID:

  9131

• Swiss Prot:

  O95831-2

• Accession Number:

  NP_665812

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDCD8.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Homeostasis

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store PDCD8 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  36 kDa, 67 kDa, 66 kDa

• Applications Notes:

  PDCD8 antibody can be used for detection of PDCD8 by ELISA at 1:62500. PDCD8 antibody can be used for detection of PDCD8 by western blot at 0.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  AIFM1

• NCBI Official Name:

  Apoptosis-inducing factor, mitochondrion-associated, 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Delettre, C., (er) J. Biol. Chem. (2006) In press.

• Other Product Names:

  PDCD8, AIF, CMT2D, CMTX4, COWCK, NADMR, NAMSD, PDCD8, COXPD6

• Tested Applications:

  ELISA, WB

• Protein ID:

  22202631

• Physical Properties:

  Liquid