NR2F2 Antibody

• Background:

  NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.

• NCBI Gene ID:

  7026

• Swiss Prot:

  P24468

• Accession Number:

  NP_066285

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR2F2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer, Signal Transduction

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1219 - MCF7 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store NR2F2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  45 kDa

• Applications Notes:

  NR2F2 antibody can be used for detection of NR2F2 by ELISA at 1:312500. NR2F2 antibody can be used for detection of NR2F2 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  NR2F2

• NCBI Official Name:

  Nuclear receptor subfamily 2, group F, member 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sato, Y., (2003) J. Clin. Endocrinol. Metab. 88 (7), 3415-3420.

• Other Product Names:

  NR2F2, ARP1, COUP-TFII, COUPTFB, MGC117452, SVP40, TFCOUP2, NF-E3, NR2F1, COUPTFII

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  14149746

• Physical Properties:

  Liquid