MAFF Antibody

• Background:

  MAFF is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. MAFF can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. MAFF may also be involved in the cellular stress response.The protein encoded by this gene is a basic leucine zipper (bZIP) transcription factor that lacks a transactivation domain. It is known to bind the US-2 DNA element in the promoter of the oxytocin receptor (OTR) gene and most likely heterodimerizes with other leucine zipper-containing proteins to enhance expression of the OTR gene during term pregnancy. The encoded protein can also form homodimers, and since it lacks a transactivation domain, the homodimer may act as a repressor of transcription. This gene may also be involved in the cellular stress response. Two transcript variants encoding the same protein have been found for this gene.

• NCBI Gene ID:

  23764

• Swiss Prot:

  Q9ULX9

• Accession Number:

  NP_690617

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish, Drosophila

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MAFF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store MAFF antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  18 kDa, 18 kDa, 18 kDa, 18 kDa, 18 kDa

• Applications Notes:

  MAFF antibody can be used for detection of MAFF by ELISA at 1:62500. MAFF antibody can be used for detection of MAFF by western blot at 5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  MAFF

• NCBI Official Name:

  V-maf avian musculoaponeurotic fibrosarcoma oncogene homolog F

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Marini, M.G., (2002) Blood Cells Mol. Dis. 29 (2), 145-158.

• Other Product Names:

  MAFF, U-MAF, hMafF

• Tested Applications:

  ELISA, WB

• Protein ID:

  23111003

• Physical Properties:

  Liquid