ILF3 Antibody

• Background:

  ILF3 may facilitate double-stranded RNA-regulated gene expression at the level of post-transcription. ILF3 can act as a translation inhibitory protein which binds to coding sequences of acid beta-glucosidase (GCase) and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 can regulate protein arginine N-methyltransferase 1 activity. ILF3 may regulate transcription of the IL2 gene during T-cell activation. It can promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA.Nuclear factor of activated T-cells (NFAT) is a transcription factor required for T-cell expression of interleukin 2. NFAT binds to a sequence in the IL2 enhancer known as the antigen receptor response element 2. In addition, NFAT can bind RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. NFAT is a heterodimer of 45 kDa and 90 kDa proteins, the larger of which is the product of this gene. The encoded protein, which is primarily localized to ribosomes, probably regulates transcription at the level of mRNA elongation. At least three transcript variants encoding three different isoforms have been found for this gene.

• NCBI Gene ID:

  3609

• Swiss Prot:

  Q12906

• Accession Number:

  NP_036350

• Host:

  Rabbit

• Reactivity:

  Human, Dog

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ILF3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cell Cycle

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1211 - HepG2 Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ILF3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  95 kDa

• Applications Notes:

  ILF3 antibody can be used for detection of ILF3 by ELISA at 1:62500. ILF3 antibody can be used for detection of ILF3 by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  ILF3

• NCBI Official Name:

  Interleukin enhancer binding factor 3, 90kDa

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Nie, Y., (2005) Mol. Cell. Biol. 25 (16), 6956-6963.

• Other Product Names:

  ILF3, CBTF, DRBF, MMP4, MPP4, NF90, NFAR, NF110, NF90a, NF90b, NFAR2, TCP80, DRBP76, NF110b, NFAR-1, TCP110, MPHOSPH4, NF-AT-90

• Tested Applications:

  ELISA, WB

• Protein ID:

  24234750

• Physical Properties:

  Liquid