TIMELESS Antibody

• Background:

  The human Timeless protein interacts with both the circadian clock protein cryptochrome 2 and with the cell cycle checkpoint proteins Chk1 and the ATR-ATRIP complex and plays an important role in the DNA damage checkpoint response. Down-regulation of Timeless in human cells seriously compromises replication and intra-S checkpoints, indicating an intimate connection between the circadian cycle and the DNA damage checkpoints that is in part mediated by the Timeless protein.

• NCBI Gene ID:

  8914

• Swiss Prot:

  Q9UNS1

• Accession Number:

  NP_003911

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TIMELESS.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Tranfected 293T Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TIMELESS antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  139 kDa

• Applications Notes:

  TIMELESS antibody can be used for detection of TIMELESS by ELISA at 1:312500. TIMELESS antibody can be used for detection of TIMELESS by western blot at 2.5 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TIMELESS

• NCBI Official Name:

  Timeless circadian clock

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sangoram, A.M., (2005) Mol. Cell. Biol. 25 (8), 3109-3116.

• Other Product Names:

  TIMELESS, TIM, TIM1, hTIM

• Tested Applications:

  ELISA, WB

• Protein ID:

  222136585

• Physical Properties:

  Liquid