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ID4 Antibody

CAT: 0223-28-779Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0223-28-779Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995) .
NCBI Gene ID
3400
Swiss Prot
P47928
Accession Number
NP_001537
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ID4.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Transcription, Cancer
Purification
Antibody is purified by protein A chromatography method.
Positive Control
Cat. No. 1211 - HepG2 Cell Lysate
Concentration
Batch dependent
Buffer
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
For short periods of storage (days) store at 4˚ C. For longer periods of storage, store ID4 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.
Calculated Molecular Weight
17 kDa
Applications Notes
ID4 antibody can be used for detection of ID4 by ELISA at 1:1562500. ID4 antibody can be used for detection of ID4 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Symbol
ID4
NCBI Official Name
Inhibitor of DNA binding 4, dominant negative helix-loop-helix protein
NCBI Organism
Homo sapiens
Background Reference 01
Yu, L., et al., (2005) Nat. Genet. 37 (3), 265-274.
Other Product Names
ID4, IDB4, bHLHb27
Tested Applications
ELISA, WB
Protein ID
4504573
Physical Properties
Liquid

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