BTF3 Antibody

• Background:

  BTF3 forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene has multiple pseudogenes.

• NCBI Gene ID:

  689

• Swiss Prot:

  P20290

• Accession Number:

  NP_001032726

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Dog, Zebrafish

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human BTF3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store BTF3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  23 kDa, 18 kDa, 18 kDa, 18 kDa

• Applications Notes:

  BTF3 antibody can be used for detection of BTF3 by ELISA at 1:312500. BTF3 antibody can be used for detection of BTF3 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  BTF3

• NCBI Official Name:

  Basic transcription factor 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Oyake, T., et al., (2005) Gene 345 (2), 271-277.

• Other Product Names:

  BTF3, NACB, BTF3a, BTF3b, BETA-NAC

• Tested Applications:

  ELISA, WB

• Protein ID:

  83641885

• Physical Properties:

  Liquid