BRF1 Antibody

• Background:

  BRF1 is one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs.

• NCBI Gene ID:

  72308

• Swiss Prot:

  Q6PED6

• Accession Number:

  AAH58112

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse BRF1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription, Cancer

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store BRF1 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  22 kDa

• Applications Notes:

  BRF1 antibody can be used for detection of BRF1 by ELISA at 1:62500. BRF1 antibody can be used for detection of BRF1 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  BRF1

• NCBI Official Name:

  BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Strausberg, R.L., et al., (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903.

• Other Product Names:

  BRF1, GTF3B, TAF3C, TAFIII90, TFIIIB90, mTFIIIB90, 2510002F24Rik

• Tested Applications:

  ELISA, WB

• Protein ID:

  34786067

• Physical Properties:

  Liquid