YAF2 Antibody

• Background:

  Yaf2 binds to MYC and inhibits MYC-mediated transactivation. Yaf2 also binds to MYCN and enhances MYCN-dependent transcriptional activation. Yaf2 increases calpain 2-mediated proteolysis of YY1 in vitro. Yaf2 is a component of the E2F6.com-1 complex, a repressive complex that methylates Lys-9 of histone H3, suggesting that it is involved in chromatin-remodeling.

• NCBI Gene ID:

  67057

• Swiss Prot:

  Q99LW6

• Accession Number:

  NP_077151

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse YAF2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by peptide affinity chromatography method.

• Positive Control:

  Cat. No. 1282 - 3T3 (NIH) Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store YAF2 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  20 kDa

• Applications Notes:

  YAF2 antibody can be used for detection of YAF2 by ELISA at 1:312500. YAF2 antibody can be used for detection of YAF2 by western blot at 0.2 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  YAF2

• NCBI Official Name:

  YY1 associated factor 2

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Kaneko, T., Gene 315, 183-192 (2003) .

• Other Product Names:

  YAF2, 2810021M11Rik

• Tested Applications:

  ELISA, WB

• Protein ID:

  40254385

• Physical Properties:

  Liquid