TFB2M Antibody

• Background:

  TFB2M is a S-adenosyl-L-methionine-dependent methyltransferase which specifically dimethylates mitochondrial 12S rRNA at the conserved stem loop. It is also required for basal transcription of mitochondrial DNA, probably via its interaction with POLRMT and TFAM. It stimulates transcription independently of the methyltransferase activity. Compared to TFB1M, it activates transcription of mitochondrial DNA more efficiently, while it has less methyltransferase activity.

• NCBI Gene ID:

  64216

• Swiss Prot:

  Q9H5Q4

• Accession Number:

  NP_071761

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TFB2M.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Tranfected 293T Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store TFB2M antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  45 kDa

• Applications Notes:

  TFB2M antibody can be used for detection of TFB2M by ELISA at 1:312500. TFB2M antibody can be used for detection of TFB2M by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  TFB2M

• NCBI Official Name:

  Transcription factor B2, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Falkenberg, M., (2002) Nat. Genet. 31 (3), 289-294.

• Other Product Names:

  TFB2M, FLJ22661, FLJ23182, Hkp1, mtTFB2

• Tested Applications:

  ELISA, WB, IHC

• Protein ID:

  11641289

• Physical Properties:

  Liquid