RRN3 Antibody

• Background:

  In mammals, growth-dependent regulation of RNA polymerase I (Pol I) transcription is mediated by RRN3, an essential initiation factor. It interacts with Pol I in the absence of template DNA, augments Pol I transcription in vivo and rescues transcription in extracts from growth-arrested cells in vitro.

• NCBI Gene ID:

  54700

• Swiss Prot:

  Q9NYV6

• Accession Number:

  NP_060897

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RRN3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Transcription

• Purification:

  Antibody is purified by protein A chromatography method.

• Positive Control:

  Cat. No. 1205 - Jurkat Cell Lysate

• Concentration:

  Batch dependent

• Buffer:

  Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  For short periods of storage (days) store at 4˚ C. For longer periods of storage, store RRN3 antibody at -20˚ C. As with any antibody avoid repeat freeze-thaw cycles.

• Calculated Molecular Weight:

  74 kDa

• Applications Notes:

  RRN3 antibody can be used for detection of RRN3 by ELISA at 1:62500. RRN3 antibody can be used for detection of RRN3 by western blot at 1.25 μ g/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

• Symbol:

  RRN3

• NCBI Official Name:

  RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Miller, G., et al., (2001) EMBO J. 20 (6), 1373-1382.

• Other Product Names:

  RRN3, TIFIA, A-270G1.2

• Tested Applications:

  ELISA, WB

• Protein ID:

  93102377

• Physical Properties:

  Liquid